Characterization and Optimization of Chitosan Production by Aspergillus terreus

Abstract The production of the amino polysaccharide (chitosan) from crustacean sources has faced many hindrances due to environmental, seasonal and noneconomic issues. On other hand, mycogenic chitosan advantages that make it suitable for medical nutritional applications over non-mycological counterparts. A number fungal isolates have been screened production, where most potent isolate genetically identified using 18S rDNA selected be focus current study. factors affecting by studied numerically optimized validated Box–Behnken design. produced collected, purified characterized degree deacetylation (DDA), molecular weight (MW), water-binding (WBC) fat-binding capacities (FBC). Results showed Aspergillus terreus (F3) was chitosan-producing with maximum productivity (2.92 g/l) at following conditions: glucose, 35.6 g/l; (NH 4 ) 2 SO , 4.6 CaCl 0.29 pH 7.9 23.2 °C 10 days. characteristics: 71.9%, DDA; 54.1165 KD, MW; 58.6%, WBC; 47.6%, FBC. features are not fully uncovered which necessitates further studies.